|
| Status |
Public on Dec 31, 2016 |
| Title |
LaminB1 DamID in HT1080 fibroblasts overexpressing NET29/TMEM120A, NET39/PPAPDC3 or NET47/TM7SF2 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Maps of genomic regions in proximity to the nuclear lamina were determined in untreated HT1080 fibroblasts and HT1080 stable cell lines expressing NET29/TMEM120A, NET39/PPAPDC3 or NET47/TM7SF2 as GFP fusions
|
| |
|
| Overall design |
The nuclear transmembrane proteins (NETs) NET29/TMEM120A, NET39/PPAPDC3 and NET47/TM7SF2 are able to reposition chromosomes towards/away from the nuclear envelope when overexpressed or knocked down in HT1080 cells. In this study we wanted to investigate peripheral chromatin changes after overexpression of these NETs.
|
| |
|
| Contributor(s) |
de las Heras JI, Zuleger N, Schirmer EC |
| Citation(s) |
28045568 |
| |
| Submission date |
Sep 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jose Ignacio de las Heras |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Trust Centre
|
| Lab |
Swann 5.12
|
| Street address |
Max Born Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3BF |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE87228 |
Transcriptional and genome organization changes in HT1080 cells after overexpression of tissue-specific nuclear transmembrane proteins (NETs) |
|
| Relations |
| BioProject |
PRJNA343797 |
| SRA |
SRP090250 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE87148_DR_n29_wt.bed.gz |
43.8 Kb |
(ftp)(http) |
BED |
| GSE87148_DR_n39_wt.bed.gz |
46.6 Kb |
(ftp)(http) |
BED |
| GSE87148_DR_n47_wt.bed.gz |
44.9 Kb |
(ftp)(http) |
BED |
| GSE87148_DR_wt_n29.bed.gz |
41.0 Kb |
(ftp)(http) |
BED |
| GSE87148_DR_wt_n39.bed.gz |
39.2 Kb |
(ftp)(http) |
BED |
| GSE87148_DR_wt_n47.bed.gz |
39.1 Kb |
(ftp)(http) |
BED |
| GSE87148_LADs_n29_15cut_sm100.bed.gz |
70.6 Kb |
(ftp)(http) |
BED |
| GSE87148_LADs_n39_15cut_sm100.bed.gz |
62.7 Kb |
(ftp)(http) |
BED |
| GSE87148_LADs_n47_15cut_sm100.bed.gz |
62.5 Kb |
(ftp)(http) |
BED |
| GSE87148_LADs_wt_15cut_sm100.bed.gz |
76.5 Kb |
(ftp)(http) |
BED |
| GSE87148_smooth_100_n29.bw |
74.3 Mb |
(ftp)(http) |
BW |
| GSE87148_smooth_100_n39.bw |
74.2 Mb |
(ftp)(http) |
BW |
| GSE87148_smooth_100_n47.bw |
74.3 Mb |
(ftp)(http) |
BW |
| GSE87148_smooth_100_wt.bw |
74.3 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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