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| Status |
Public on May 02, 2017 |
| Title |
Genome-wide profiling of H3K27me3 in Drosophila primary spermatocytes. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
YFP-tagged primary spermatocytes were sorted by FACS, then ChIP together with microarray analysis was used to generate a genome-wide map for the H3K27me3 histone mark.
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| Overall design |
We performed two biological replicates with a Cy3/Cy5 dye swap. Input chromatin was used as the reference control to assay ChIP enrichment.
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| Contributor(s) |
El-Sharnouby SM, Fischer B, White RA |
| Citation(s) |
28282436 |
| |
| Submission date |
Aug 11, 2016 |
| Last update date |
Jul 12, 2017 |
| Contact name |
Rob White |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Cambridge
|
| Department |
Physiology, Development and Neuroscience
|
| Street address |
Downing Street
|
| City |
Cambridge |
| ZIP/Postal code |
CB23DY |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL15057 |
NimbleGen Drosophila melanogaster Whole Genome 2.1M tiling array [DM_5_Catalog_tiling_HX1; DesignID 6725] |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE85504 |
Regions of very low H3K27me3 partition the Drosophila genome into topological domains |
|
| Relations |
| BioProject |
PRJNA338724 |
