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| Status |
Public on Nov 20, 2016 |
| Title |
Dynamic regulation of chromatin signatures during Drosophila embryonic glial differentiation [ChIP-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Employ affinity purification approach to capture different stages of glial-specific information on gene expression, nucleosome occupancy and histone modifications (HMs) in Drosophila melanogaster embryo
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| Overall design |
Cell type-specific nucleosome and histone modification maps were generated from GBs and glias nuclei, which isolated from 5–7h-old gcm-Gal4,UAS-mCDGFP/cyo;UAS-3xFLAG-blrp-mCherry-RanGap and 12–14h-old repo-Gal4,UAS-3xFLAG-blrp-mCherry-RanGap/Tm3B D. melanogaster embryos by the method INTACT (Steinner et al., 2012). We determinated nucleosome occupancy in these nuclei by micrococcal nuclease sequencing (MNase-Seq). We also performed ChIP-Seq (chromatin immunoprecipitation and sequencing) for five histone modifications (H3K4me3, H3K4me1,H3K9ac, H3K27ac, and H3K27me3). Sequencing was performed on Illumina HiSeq 2000 using signle end protocol.
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| Contributor(s) |
Ye Y, Jiang C |
| Citation(s) |
27634414, 31195140 |
| |
| Submission date |
Jun 15, 2016 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Youqiong Ye |
| E-mail(s) |
[email protected]
|
| Phone |
+8613661565591
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| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Street address |
280 Chongqing south road
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE83377 |
Dynamic regulation of chromatin signatures during Drosophila embryonic glial differentiation |
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| Relations |
| BioProject |
PRJNA325733 |
| SRA |
SRP076594 |
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