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| Status |
Public on Jul 22, 2016 |
| Title |
The Nrd1-like protein Seb1 coordinates co-transcriptional 3’ end processing and polyadenylation site selection |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Other
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| Summary |
Termination of RNAPII transcription is associated with RNA 3’ end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in S. cerevisiae do not rely on RNA cleavage for termination, but instead terminate via a pathway that requires the Nrd1-Nab3-Sen1 (NNS) complex. Here we show that the S. pombe ortholog of Nrd1, Seb1, does not function in NNS-like termination, but promotes polyadenylation site selection of coding and noncoding genes. We found that Seb1 associates with 3’ end processing factors, is enriched at the 3’ end of genes, and binds RNA motifs downstream of cleavage sites. Importantly, a deficiency in Seb1 resulted in widespread changes in 3’ UTR length as a consequence of increased alternative polyadenylation. Given that Seb1 levels affected the recruitment of conserved 3’ end processing factors, our findings indicate that the conserved RNA-binding protein Seb1 co-transcriptionally controls alternative polyadenylation.
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| Overall design |
Two biological replicates of Seb1 and Control (parental strain) CRAC experiments
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| Contributor(s) |
Granneman S, Bachand F |
| Citation(s) |
27401558 |
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| Submission date |
Jun 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sander Granneman |
| E-mail(s) |
[email protected]
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| Organization name |
University of Edinburgh
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| Department |
Centre for Synthetic and Systems Biology
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| Lab |
Granneman lab
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| Street address |
Mayfield Road, Kings Buildings, Waddington building, room 3.06
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| City |
Edinburgh |
| ZIP/Postal code |
EH9 3JD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17225 |
Illumina HiSeq 2500 (Schizosaccharomyces pombe) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA324654 |
| SRA |
SRP076220 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE82326_RAW.tar |
1.6 Mb |
(http)(custom) |
TAR (of GTF) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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