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| Status |
Public on Oct 17, 2016 |
| Title |
Small-RNA transcriptome sequencing of individual cells |
| Organisms |
Homo sapiens; synthetic construct |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Several classes of small RNAs have been described, however to molecularly profile them require large numbers of cells. Here, we developed a method for single-cell small-RNA sequencing that we applied to naïve and primed human embryonic stem cells and to cancer cells. Single-cell profiling of microRNAs and fragments of tRNAs and snoRNAs revealed that, in particular, microRNAs have unrecognized potential to separate cell types and states.
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| |
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| Overall design |
Small-RNA transcriptome sequencing (Small-seq) is a method designed to capture all small RNAs at single cells
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| |
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| Contributor(s) |
Abdullayev I, Faridani OR, Sandberg R |
| Citation(s) |
27798564 |
| |
| Submission date |
May 10, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ilgar Abdullayev |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institutet
|
| Department |
Cell and Molecular Biology
|
| Lab |
Rickard Sandberg's group ([email protected])
|
| Street address |
Nobels väg 3
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL15228 |
Illumina HiSeq 2000 (synthetic construct) |
|
| Samples (539)
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| Relations |
| BioProject |
PRJNA321192 |
| SRA |
SRP074776 |
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