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Series GSE8070 Query DataSets for GSE8070
Status Public on Jun 09, 2007
Title Expression profiling of pancreas development
Organism Mus musculus
Experiment type Expression profiling by array
Summary Development of the pancreas from the endoderm is initiated at embryonic day 9 of mouse development and over the following days several different cell types develop from pancreas progenitor cells. A distinct phase of pancreas development, known as the secondary transition, is initiated at day 13 of development and one of the key features of this transition is a massive increase in the number of mature endocrine cells. To study gene expression in pancreas during the secondary transition we performed high-density oligonucleotide microarray experiments on dorsal pancreas tissue isolated from NMRI embryos on consecutive days from e12.5 to e16.5.
Keywords: time course
 
Overall design Dorsal pancreata were isolated from embryos at embryonic day 12.5, 13.5, 14.5, 15.5, and 16.5 and pooled litter-wise prior to total RNA extraction. From each pool, two independent labelling reactions were made, and each sample was hybridized to the entire Murine Genome U74 version 2 chip set (A, B, and C).
 
Contributor(s) Petri A
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Submission date Jun 08, 2007
Last update date Feb 18, 2018
Contact name Andreas Petri
E-mail(s) [email protected]
Organization name Novo Nordisk A/S
Department Molecualr Genetics
Street address Novo Nordisk Park D6 2.154
City Maaloev
ZIP/Postal code 2760
Country Denmark
 
Platforms (3)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL82 [MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array
GPL83 [MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array
Samples (30)
GSM199443 Pancreas at e12.5, Chip A, rep 1
GSM199444 Pancreas at e12.5, Chip A, rep 2
GSM199445 Pancreas at e13.5, Chip A, rep 1
Relations
BioProject PRJNA100907

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8070_RAW.tar 79.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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