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| Status |
Public on Oct 04, 2016 |
| Title |
Alteration of gene expression profile of cerebelllar neuron-enriched cultures from neonatal rats by nicotine, acetamiprid, or imidacloprid treatment [nicotine] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Neonicotinoids are considered safe because of their low affinities to mammalian nicotinic acetylcholine receptors (nAChRs) relative to insect nAChRs. However, because of importance of nAChRs in mammalian brain development, there remains a need to establish the safety of chronic neonicotinoid exposures with regards to children’s health. Here we examined the effects of long-term (14 days) and low dose (1 μM) exposure of neuron-enriched cultures from neonatal rat cerebellum to nicotine and two neonicotinoids: acetamiprid and imidacloprid. Immunocytochemistry revealed no differences in the number or morphology of immature neurons or glial cells in any group versus untreated control cultures. However, a slight disturbance in Purkinje cell dendritic arborization was observed in the exposed cultures. Next we performed transcriptome analysis on total RNAs using microarrays, and identified significant differential expression (p < 0.05, q < 0.05, ≥1.5 fold) between control cultures versus nicotine-, acetamiprid-, or imidacloprid-exposed cultures in 34, 48, and 67 genes, respectively. Common to all exposed groups were nine genes essential for neurodevelopment, suggesting that chronic neonicotinoid exposure alters the transcriptome of the developing mammalian brain in a similar way to nicotine exposure. Our results highlight the need for further careful investigations into the effects of neonicotinoids in the developing mammalian brain.
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| Overall design |
Using cerebellar neuron-enriched cultures from neonatal rats (1 day), chronic exposure of nicotine (1 μM), imidacloprid (1 μM), or acetamiprid (1 μM) was examined from 2 days to 16 days in vitro. After 16 days in vitro, samples (n=6 /each group) of cerebellar neuron-enriched cultures including control and each treatment were taken for gene expression analysis.
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| Web link |
http://www.mdpi.com/1660-4601/13/10/987
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| Contributor(s) |
Kimura-Kuroda J, Hayashi M |
| Citation(s) |
27782041 |
| |
| Submission date |
Apr 25, 2016 |
| Last update date |
Dec 21, 2016 |
| Contact name |
Junko Kimura-Kuroda |
| E-mail(s) |
[email protected]
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| Organization name |
Tokyo Metropolitan Institute of Medical Science
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| Department |
Department of Brain Development and Neural Regeneration
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| Street address |
2-1-6, Kamikitazawa
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
156-8506 |
| Country |
Japan |
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| Platforms (1) |
| GPL14746 |
Agilent-028282 Whole Rat Genome Microarray 4x44K v3 (Probe Name version) |
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| Samples (12)
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| GSM2132047 |
Cerebellar neuron enriched culture 16days control for nicotine rep1 |
| GSM2132048 |
Cerebellar neuron enriched culture 16days control for nicotine rep2 |
| GSM2132049 |
Cerebellar neuron enriched culture 16days control for nicotine rep3 |
| GSM2132050 |
Cerebellar neuron enriched culture 16days control for nicotine rep4 |
| GSM2132051 |
Cerebellar neuron enriched culture 16days control for nicotine rep5 |
| GSM2132052 |
Cerebellar neuron enriched culture 16days control for nicotine rep6 |
| GSM2132053 |
Cerebellar neuron enriched culture 16days nicotine rep1 |
| GSM2132054 |
Cerebellar neuron enriched culture 16days nicotine rep2 |
| GSM2132055 |
Cerebellar neuron enriched culture 16days nicotine rep3 |
| GSM2132056 |
Cerebellar neuron enriched culture 16days nicotine rep4 |
| GSM2132057 |
Cerebellar neuron enriched culture 16days nicotine rep5 |
| GSM2132058 |
Cerebellar neuron enriched culture 16days nicotine rep6 |
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| This SubSeries is part of SuperSeries: |
| GSE80656 |
Alteration of gene expression profile of cerebelllar neuron-enriched cultures from neonatal rats by nicotine, acetamiprid, or imidacloprid treatment |
|
| Relations |
| BioProject |
PRJNA319577 |
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