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| Status |
Public on Apr 08, 2016 |
| Title |
The Forkhead box transcription factor FOXM1 is required for the maintenance of cell proliferation and protection against oxidative stress in human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Human embryonic stem cells (hESCs) exhibit unique cell cycle structure, self-renewal and pluripotency. The Forkhead box transcription factor M1 (FOXM1) is critically required for the maintenance of pluripotency in mouse embryonic stem cells and mouse embryonal carcinoma cells, but its role in hESCs remains unclear. Here, we show that FOXM1 expression was enriched in undifferentiated hESCs and was regulated in a cell cycle-dependent manner with peak levels detected at the G2/M phase. Expression of FOXM1 did not correlate with OCT4 and NANOG during in vitro differentiation of hESCs. Importantly, knockdown of FOXM1 expression led to aberrant cell cycle distribution with impairment in mitotic progression but showed no profound effect on the undifferentiated state. Interestingly, FOXM1 depletion sensitized hESCs to oxidative stress. Moreover, genome-wide analysis of FOXM1 targets by ChIP-seq identified genes important for M phase including CCNB1 and CDK1, which were subsequently confirmed by ChIP and RNA interference analyses. Further peak set comparison against a differentiating hESC line and a cancer cell line revealed substantial difference in the genomic binding profile of FOXM1 in hESCs. Taken together, our findings provide the first evidence to support FOXM1 as important regulator of cell cycle progression and defense against oxidative stress in hESCs.
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| Overall design |
Examination of genomic binding profile of FOXM1 in human embryonic stem cells by ChIP-seq, using Illumina HiSeq 2000
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| Contributor(s) |
Kwok C, Qin Y |
| Citation(s) |
27062359 |
| |
| Submission date |
Mar 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kwok Ming YAO |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Hong Kong
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| Department |
School of Biomedical Sciences
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| Street address |
L3-69, Laboratory Block, 21 Sassoon Road
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| City |
Hong Kong |
| ZIP/Postal code |
NA |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA316720 |
| SRA |
SRP072515 |
