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| Status |
Public on Mar 16, 2016 |
| Title |
Type-I interferon inducible genes 26 weeks after mCMV infection in Siglec-H wt and Siglec-H ko mice and their non infected controls |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Type I interferons are critical anti-viral cytokines during virus infections and have also been implicated in the pathogenesis of systemic lupus erythematosus (SLE). The secretion of type I interferon of pDCs is modulated by Siglec-H, a DAP12 associated receptor on pDCs. We showed that Siglec-H deficient pDCs produce more of the type I interferon IFN-α in vitro and that Siglec-H ko mice produce more IFN-α after murine cytomegalovirus (mCMV) infection in vivo, leading to efficient clearance of the virus. Furthermore, ageing Siglec-H ko mice showed a mild form of systemic autoimmunity. In contrast, Siglec-H ko mice developed a severe form of systemic lupus-like autoimmune disease with strong kidney nephritis several weeks after a single mCMV infection. This induction of systemic autoimmune disease after virus infection in Siglec-H ko mice was accompanied by a type I interferon signature and fully dependent on type I interferon signaling. These results show that Siglec-H normally serves as modulator of type I interferon responses after infection with a persistent virus and thereby prevents induction of autoimmune disease.
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| Overall design |
For microarray experiments gene expression profiles of total splenic cells from two wt and Siglec-H ko mice 26 weeks after infection with luciferase expressing murine Cytomegalovirus (5x105 pfu) or from two uninfected wt and Siglec-H ko control mice were analyzed
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| Contributor(s) |
Schmitt H, Sell S, Koch J, Sonnewald S, Daniel C, Winkler TH, Nitschke L |
| Citation(s) |
27377589 |
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| Submission date |
Mar 15, 2016 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Heike Schmitt |
| E-mail(s) |
[email protected]
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| Phone |
+4991318528529
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| Organization name |
Friedrich Alexander University
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| Department |
Biology
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| Lab |
Prof. Dr Lars Nitschke
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| Street address |
Staudtstraße 5
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| City |
Erlangen |
| ZIP/Postal code |
91058 |
| Country |
Germany |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA315273 |
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