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| Status |
Public on Feb 10, 2017 |
| Title |
Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Pulsed ecdysone signaling remodels cell cycle dynamics, causing distinct primary and secondary cell cycle arrests, similar to those observed in the wing during metamorphosis.
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| Overall design |
This was a timecourse transcriptome analysis of the Drosophila pupal wing during metamorphosis by RNAseq. Timepoints include the proliferative wandering L3 larval stage (-10h APF), the temporary G2 arrest (6h APF) the final cell cycle (18h APF) the permanent cell cycle arrest (24h APF), and stages of terminal differentiation including vein differentiation, wing hair formation and cuticle protein production (36 and 44h APF)
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| |
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| Contributor(s) |
Guo Y, Flegel K, Norman KL, Kumar J, McKay DJ, Buttitta LA |
| Citation(s) |
27737823, 29126440 |
| |
| Submission date |
Feb 03, 2016 |
| Last update date |
May 24, 2019 |
| Contact name |
Daniel McKay |
| E-mail(s) |
[email protected]
|
| Organization name |
University of North Carolina, Chapel Hill
|
| Department |
Biology and Genetics
|
| Lab |
McKay
|
| Street address |
250 Bell Tower Drive
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA310853 |
| SRA |
SRP069300 |
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