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| Status |
Public on Jan 28, 2016 |
| Title |
Gene expression profiles of B cells under various conditions |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To validate the functions that iron might play in B cell proliferation and function we used deferoxamine (DFO, a widely used iron chelator) to create an iron-deficient environment for cell culture in vitro.
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| Overall design |
We measured B cell proliferative responses to mitogenic stimuli under several conditions, primary splenic B cells cultured in normal medium, or in the presence of DFO (deferoxamine, 20uM) ,or DFO accompanied with an additional supplement of FAC (Ferric ammonium citrate, 100uM) or FAC(100uM) alone in the medium, unstimulated or stimulated anti-IgM(10ug/ml) or LPS (2ug/ml) for 48h.
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| Contributor(s) |
Jiang Y |
| Citation(s) |
31270335 |
| |
| Submission date |
Jan 27, 2016 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Yuhang Jiang |
| E-mail(s) |
[email protected]
|
| Organization name |
Shanghai Institutes for Biological Sciences, CAS
|
| Department |
Institute of Health Sciences
|
| Lab |
Laboratory of Immunoregulation and Tolerance
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA310002 |
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