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| Status |
Public on Mar 31, 2016 |
| Title |
Expression data from non-small cell lung cancer cell line DV90 after Bromodomain and extra terminal domain (BET) inhibitor JQ1 treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Bromodomain and extra terminal domain (BET) inhibition reduces occupancy of BET-family proteins at promoter and enhancer sites resulting in changes in the transcription of specific genes.
We used microarray profiling to investigate the transcriptional changes induced by BET inhibitor JQ1 treatment in DV90 cells to identify the underlying changes of gene regulation that lead to JQ1 sensitivity.
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| Overall design |
DV90 cells (JQ1 sensitive non-small cell lung cancer cell line) were treated with 135 nM (IC50) or 785 nM (IC90) of JQ1 for 4h and 24h. DMSO treated controls served as reference and at least four replicates per condition were collected. RNA was extracted and hybridized to Affymetrix HuGene-2.1ST microarrays to identify treatment induced transcriptional changes.
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| Contributor(s) |
Klingbeil O, Lesche R |
| Citation(s) |
27607580 |
| |
| Submission date |
Dec 14, 2015 |
| Last update date |
Dec 11, 2017 |
| Contact name |
Ralf Lesche |
| E-mail(s) |
[email protected]
|
| Organization name |
Bayer Pharma AG
|
| Street address |
Muellerstr 178
|
| City |
Berlin |
| ZIP/Postal code |
13342 |
| Country |
Germany |
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| Platforms (1) |
| GPL17692 |
[HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA305859 |
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