|
| Status |
Public on Nov 20, 2015 |
| Title |
Knockdown of Polyhomeotic in Drosophila Kc cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this experiment, we used RNAi targeting both copies of the gene polyhomeotic (ph-p and ph-d) to knockdown expression of this factor in Drosophila Kc167 cells. We observed substantial changes to the structure of genomic loci bound by Polycomb-group proteins as assayed by single-molecule based super-resolution imaging with STORM. We also observed a substantial de-repression of many genes which reside in Polycomb-bound domains, as assayed with RNA-seq.
|
| |
|
| Overall design |
mRNA profiles from Drosophila Kc167 cells with and without knockdown the genes ph-p and ph-d were examined in duplicate.
|
| |
|
| Contributor(s) |
Boettiger A, Zhuang X |
| Citation(s) |
26760202 |
| |
| Submission date |
Nov 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiaowei Zhuang |
| E-mail(s) |
[email protected]
|
| Phone |
617-384-9818
|
| Organization name |
Harvard University
|
| Department |
Chemistry and Chemical Biology
|
| Lab |
Zhuang
|
| Street address |
12 Oxford St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA302312 |
| SRA |
SRP066261 |
Less...
More...