|
| Status |
Public on Sep 25, 2016 |
| Title |
Expression data of Met-low and Met-high B16-F10 malignant melanoma cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
B16-F10 malignant mouse melanoma cells have been frequently used as highly metastatic cells. Based on heterogenous cell surface expression of Met/HGF (hepatocyte growth factor) receptor in B16-F10 cells, the cells were divided into Met-low and Met-high cells by flow cytometry and these populations were subjected to microarray analysis. Met-low and Met-high cells showed different expression profiles in genes involved characteristics of tumors, including stem cell maintenance, pigmentation, and angiogenesis.
|
| |
|
| Overall design |
Total RNA was prepared respectively from Met-low and Met-high cells cultured for 3 days after cell sorting. RNA samples (n=2 for each groups) were subjected to microarray analysis, using the Whole Mouse Genome (44,000) Oligo Microarray (Agilent Technologies).
|
| |
|
| Contributor(s) |
Adachi E, Sakai K, Nishiuchi T, Matsumoto K |
| Citation(s) |
27683122 |
| |
| Submission date |
Jun 10, 2015 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Kunio Matsumoto |
| E-mail(s) |
[email protected]
|
| Organization name |
Kanaawa University
|
| Street address |
Kakuma
|
| City |
Kanazawa |
| ZIP/Postal code |
920-1192 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
|
| Samples (4)
|
| GSM1708070 |
RNA from Met-low cells of B16-F10 cell line_1 |
| GSM1708071 |
RNA from Met-low cells of B16-F10 cell line_2 |
| GSM1708072 |
RNA from Met-high cells of B16-F10 cell line_1 |
| GSM1708073 |
RNA from Met-high cells of B16-F10 cell line_2 |
|
| Relations |
| BioProject |
PRJNA286310 |
Less...
More...