|
| Status |
Public on Apr 21, 2015 |
| Title |
Sex bias and maternal contribution to gene expression divergence in Drosophila blastoderm embryos |
| Organisms |
Drosophila melanogaster; Drosophila pseudoobscura; Drosophila virilis; Drosophila yakuba |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Early embryogenesis is a unique developmental stage where genetic control of development is handed off from mother to zygote. Yet the contribution of this transition to the evolution of gene expression is poorly understood. Here we study two aspects of gene expression specific to early embryogenesis in Drosophila: sex-biased gene expression prior to the onset of canonical X chromosomal dosage compensation, and the contribution of maternally supplied mRNAs. We sequenced mRNAs from individual unfertilized eggs, precisely staged and sexed blastoderm embryos and compared levels between D. melanogaster, D. yakuba, D. pseudoobscura and D. virilis. First, we find that mRNA content is highly conserved for a given stage and that studies relying on pooled embryos may systematically overstate the degree of gene expression divergence. Unlike studies done on larvae and adults, where most species show a larger proportion of genes with male-biased expression, we find that transcripts in Drosophila embryos are largely female-biased in all species, likely due to incomplete dosage compensation prior to the activation of the canonical dosage compensation mechanism. The divergence of sex-biased gene expression across species is observed to be often due to a decrease of expression, the most drastic example of which is the overall reduction of male expression from the neo-X chromosome in D. pseudoobscura, leading to a pervasive female-bias on this chromosome. We see no evidence for a faster evolution of expression on the X chromosome in embryos (no “faster X” effect), unlike in adults and contrary to a previous study on pooled non-sexed embryos. Finally, we find that most genes are conserved in regard to their maternal or zygotic origin of transcription, and present evidence that differences in maternal contribution to the blastoderm transcript pool may be due to species-specific divergence of transcript degradation rates
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| Overall design |
We sequenced mRNA from D. melanogaser, D. yakuba, D. pseudoobscura and D. virilis single unfertilized eggs (1 to 2 per species) and from both single female and male embryos (3 per sex per species).
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| |
|
| Contributor(s) |
Paris M, Villalta JE, Eisen MB, Lott SE |
| Citation(s) |
26485701 |
| |
| Submission date |
Apr 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mathilde Paris |
| Organization name |
CNRS
|
| Department |
IGFL
|
| Street address |
Faculte des sciences de Luminy, case 907
|
| City |
LYON |
| ZIP/Postal code |
69007 |
| Country |
France |
| |
|
| Platforms (4)
|
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL13307 |
Illumina HiSeq 2000 (Drosophila yakuba) |
| GPL13310 |
Illumina HiSeq 2000 (Drosophila pseudoobscura) |
| GPL13313 |
Illumina HiSeq 2000 (Drosophila virilis) |
|
| Samples (31)
|
|
| Relations |
| BioProject |
PRJNA281640 |
| SRA |
SRP057462 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68062_Gene_CLASS_after_FPKM_normalization.txt.gz |
108.8 Kb |
(ftp)(http) |
TXT |
| GSE68062_Gene_CLASS_after_Q_normalization.txt.gz |
109.9 Kb |
(ftp)(http) |
TXT |
| GSE68062_Gene_CLASS_after_TMM_normalization.txt.gz |
109.2 Kb |
(ftp)(http) |
TXT |
| GSE68062_Gene_CLASS_after_p50_normalization.txt.gz |
108.2 Kb |
(ftp)(http) |
TXT |
| GSE68062_Gene_CLASS_after_p75_normalization.txt.gz |
108.3 Kb |
(ftp)(http) |
TXT |
| GSE68062_Gene_CLASS_after_p95_normalization.txt.gz |
109.0 Kb |
(ftp)(http) |
TXT |
| GSE68062_Gene_abundances_after_FPKM_normalization.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
| GSE68062_Gene_abundances_after_Q_normalization.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| GSE68062_Gene_abundances_after_TMM_normalization.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
| GSE68062_Gene_abundances_after_p50_normalization.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
| GSE68062_Gene_abundances_after_p75_normalization.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
| GSE68062_Gene_abundances_after_p95_normalization.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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