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| Status |
Public on May 12, 2015 |
| Title |
The ChIP-Sequence data of STAT92E in Drosophila adult intestines |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
JAK/STAT pathway plays important roles in controlling Drosophila intestinal homeostasis and regulating the ISC proliferation and differentiation. However,the downstream targets of its transcription factor-STAT92E remain largely unknown.To further identify the regualtory mechanisms of the JAK/STAT pathway in controlling intestinal homeostasis,we performed the ChIP-Seq assay with mouse raised STAT92E antibody using JAK/STAT signaling highly activated adult intestines.Through the ChIP assay, we have identified over 1000 significant peaks (p<0.01) around the putative targets.The well-characterized JAK/STAT downstream targets including Domeless,Socs36E,STAT92E and chinmo were identified in our ChIP assay,indicating that our experiment is workable to identify novel JAK/STAT downstream targets in adult intestines.This work will provide insights into our understanding of regulatory mechanisms of JAK/STAT signaling during Drosophila intestinal development.
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| Overall design |
Identify the ChIP peaks of STAT92E antibody using JAK/STAT signaling highly actived Drosophila adult intestines, compared with input libaray as the control
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| Contributor(s) |
Zhang Y, Ren W, Cai W, Lin X |
| Citation(s) |
25923769 |
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| Submission date |
Mar 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Wanshi Cai |
| E-mail(s) |
[email protected]
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| Organization name |
CHINESE ACADEMY OF SCIENCES
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| Department |
INSTITUTE OF ZOOLOGY
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| Lab |
State Key Laboratory of Biomembrane and Membrane Biotechnology
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| Street address |
1 Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA279555 |
| SRA |
SRP056621 |
