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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 25, 2015 |
| Title |
Effect of 90Sr internal emitter on gene expression in mouse blood |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Background The radioactive isotope Strontium-90 (90Sr) may be released as a component of fallout from nuclear accidents, or in the event of a radiological incident such as detonation of an improvised nuclear device, and if ingested poses a significant health risk to exposed individuals. In order to better understand the response to 90Sr, using an easily attainable and standard biodosimetry sample fluid, we analyzed the global transcriptomic response of blood cells in an in vivo model system.
Methods We injected C57BL/6 mice with a solution of 90SrCl2 and followed them over a 30-day period. At days 4, 7, 9, 25 and 30, we collected blood and isolated RNA for microarray analyses. These days corresponded to target doses in a range from 1 to 5 Gy. We investigated changes in mRNA levels using microarrays, and changes in specific microRNA (miRNA) predicted to be involved in the response using qRT-PCR.
Results We identified 8082 differentially expressed genes in the blood of mice exposed to 90Sr compared with controls. Common biological functions were affected throughout the study, including apoptosis of B and T lymphocytes, and atrophy of lymphoid organs. Cellular functions such as RNA degradation and lipid metabolism were also affected during the study. The broad down regulation of genes observed in our study suggested a potential role for miRNA in gene regulation. We tested candidate miRNAs, mmu-miR-16, mmu-miR-124, mmu-miR-125 and mmu-mir-21; and found that all were induced at the earliest time point, day 4.
Conclusions Our study is the first to report the transcriptomic response of blood cells to the internal emitter 90Sr in mouse and a possible role for microRNA in gene regulation after 90Sr exposure. The most dramatic effect was observed on gene expression related to B-cell development and RNA maintenance. These functions were affected by genes that were down regulated throughout the study, suggesting severely compromised antigen response, which may be a result of the deposition of the radioisotope proximal to the hematopoietic compartment in bone.
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| Overall design |
Radiation induced gene expression in mouse blood was measured at 4, 7, 9, 25 and 30 days after 90Sr injection. Six independent experiments were performed at each time point.
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| Contributor(s) |
Ghandhi SA, Amundson SA |
| Citation(s) |
26251171 |
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| Submission date |
Jan 08, 2015 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Shanaz Adi Ghandhi |
| E-mail(s) |
[email protected]
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| Phone |
212-3053911
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| Organization name |
Columbia University Medical Center
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| Department |
Center for Radiological Research
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| Lab |
VC11-237
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| Street address |
630, W 168th street
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (56)
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| Relations |
| BioProject |
PRJNA271849 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64775_RAW.tar |
499.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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