|
| Status |
Public on Nov 14, 2014 |
| Title |
Transcriptome-wide modulation of splicing by the exon junction complex |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report that knockdown of EJC core proteins, eIF4A3, Y14, Magoh, causes a transcript-wide changes in alternative splicing, as well as some transcriptional changes. These changes are specific to EJC core proteins, and KD of UPF1 protein caused different sets of alterantive splicing changes. These changes are linked to the rate of transcription.
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| |
|
| Overall design |
Examination of 4 different knockdown, as well as GFP knockdown in HeLa cells, 2 replicates each condition.
|
| |
|
| Contributor(s) |
Wang Z, Murigneux V, Le Hir H |
| Citation(s) |
25476502 |
| |
| Submission date |
Nov 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Herve LE HIR |
| E-mail(s) |
[email protected]
|
| Organization name |
IBENS
|
| Department |
Functional Genomics
|
| Lab |
Le Hir lab
|
| Street address |
46 rue d'Ulm
|
| City |
PARIS |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA266584 |
| SRA |
SRP049611 |
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