Expression profiling by high throughput sequencing
Summary
We performed mRNA transcriptional profiling on 99 hemizygotic lines (Df/+) from the DrosDel project covering 68% of chromosome 2L, in order to understand how changes in gene copy number affect overall transcriptome.
Overall design
We performed RNA-Seq analysis on 396 pools of 15-25 adult flies each. Samples include males or females from 99 different genotypes in duplicate. Differential gene expression was analyzed separately for each sex, by comparing each genotype with the remaining 98.
Hangnoh Lee, Dong-Yeon Cho, Cale Whitworth, Robert Eisman, Melissa Phelps, John Roote, Thomas Kaufman, Kevin Cook, Steven Russell, Teresa M Przytycka, Brian Oliver. Genome-wide expression profiling Drosophila melanogaster deficiency heterozygotes reveals diverse genomic responses. bioRxiv doi: http://dx.doi.org/10.1101/032920 Dong-Yeon Cho, Hangnoh Lee, Damian Wojtowicz, Steven Russell, Brian Oliver, Teresa M Przytycka. Interplay between copy number, dosage compensation and expression noise in Drosophila. bioRxiv doi: https://doi.org/10.1101/041038
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