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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 27, 2014 |
| Title |
Med23-dependent gene expression programs in T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To analysis TCR induced Med23-dependent gene expression programs in T cells, we have employed microarray expression profiling as a discovery platform to identify genes with the potential to be regulated by Med23 in unstimulated or stimulated T cells. Mice CD4 T cells from wildtype and Med23 knockout mice were left unstimulated or stimulated for 6 hr, and gene expression difference was identified among four samples. Expression of seven genes (Med23, IFNg, IL2, Egr2, c-Fos, c-Jun and Foxp1) was validated in the same RNA samples by real-time PCR.
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| Overall design |
TCR induced Med23-dependent gene expression was measured after unstimulation or stimulation for 6 hr in wildtype and Med23 knockout CD4 T cells.
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| Contributor(s) |
Liu X, Sun Y, Zhu X |
| Citation(s) |
25301163 |
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| Submission date |
Aug 26, 2014 |
| Last update date |
Jan 08, 2018 |
| Contact name |
Yang Sun |
| Organization name |
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
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| Street address |
Yueyang Road 320
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA259533 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60779_Raw_Intensity.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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