|
| Status |
Public on Oct 20, 2014 |
| Title |
Cooperativity, specificity and evolutionary stability of Polycomb targeting in Drosophila |
| Organisms |
Drosophila melanogaster; Drosophila pseudoobscura; Drosophila simulans; Drosophila virilis; Drosophila yakuba |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the evolutionary behaviour of Polycomb group proteins, their recruitment factors and their underlying sequences by performing ChIP-seq analysis in 4-5 different Drosophila species. We demonstrate an extremely high conservation of Polycomb repressive domains across Drosophila species We validate few cases of PRE divergence that shows that cis-driven PRE evolution is a rare event. We further show that PHO recruitment to Polycomb domains is evolutionarily robust to motif changes and that PRC1 stabilizes binding of its key recruiter
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| |
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| Overall design |
ChIP-seq analysis of histone marks and chromatin associated factors across 4-5 Drosophila species
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| |
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| Contributor(s) |
Schuettengruber B, Elkayam NO, Sexton T, Entrevan M, Stern S, Thomas A, Yaffe E, Parrinello H, Tanay A, Cavalli G |
| Citation(s) |
25284790 |
| |
| Submission date |
Aug 14, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Bernd Schuettengruber |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute de Génétique Humaine
|
| Lab |
Cavalli
|
| Street address |
141, rue de la cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34396 |
| Country |
France |
| |
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| Platforms (6)
|
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL15872 |
Illumina Genome Analyzer IIx (Drosophila yakuba) |
| GPL15873 |
Illumina Genome Analyzer IIx (Drosophila virilis) |
| GPL17484 |
Illumina Genome Analyzer IIx (Drosophila simulans) |
| GPL17883 |
Illumina Genome Analyzer IIx (Drosophila pseudoobscura) |
|
| Samples (70)
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|
| Relations |
| BioProject |
PRJNA258256 |
| SRA |
SRP045520 |
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