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| Status |
Public on Jan 03, 2015 |
| Title |
Identifying interferon-stimulated genes enriched in multiple tissues of Irgm1-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We observed enhanced epithelial proliferation secondary to elevated Type I IFNs in multiple tissues of Irgm1-/- mice. We discovered that Type I IFNs signaled through macrophages to promote this epithelial hyperproliferation. To determine candidate factors that could play a role in this process, we performed whole genome microarray analysis of salivary gland, small intestine, and isolated colonic macrophages of Irgm1-/- mice and C57BL/6 WT controls. Genes enriched in at least 2 of the 3 sample sources were then screened for the ability to augment epithelial proliferation in vitro.
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| Overall design |
RNA was extracted from three (small intestine and isolated colonic macrophage samples) or four (salivary gland samples) mice of each genotype (Irgm1-/- and WT). Both male and female six to nine week-old mice were used.
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| Contributor(s) |
Sun L, Stappenbeck TS |
| Citation(s) |
25482432 |
| |
| Submission date |
Jul 21, 2014 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Lulu Sun |
| Organization name |
Washington University in St. Louis
|
| Department |
Pathology and Immunology
|
| Lab |
Thaddeus S. Stappenbeck
|
| Street address |
4940 Parkview Place, 1020 CSRB-NTA
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA255751 |
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