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| Status |
Public on Feb 26, 2015 |
| Title |
Transcriptome analysis of CD4+ T cells in experimental autoimmune encephalitomyelitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In this study we determined whole genome gene expression of murine naive CD4+ T cells, in vitro differentiated Th17 cells, and CD4+ T cells isolated from experimental autoimmune encephalitomyelitis (EAE)-affected animals either after adoptive transfer of Th17 cells or after immunization with MOG35-55-peptide. The overall goal was to identify candidate genes involved in T cell pathology, encephalitogenicity and plasticity. These findings could then be correlated to multiple sclerosis pathology.
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| Overall design |
Naive CD4+ T cells were isolated from B6.2d2 transgenic mice with MOG-specific T cell receptors and differentiated in vitro into Th17 cells. These Th17 cells were adoptively transferred into lymphopenic RAG1-/- mice to induce EAE. Further, EAE was induced by immunizing wild-type C57BL/6 mice with MOG35-55 peptide. RNA was extracted from naive CD4+ T cells, Th17 cells, and from CD4+ T cells isolated from the CNS of EAE-affected mice for gene expression analysis. Replicates from three independent experiments were analyzed.
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| Contributor(s) |
Hoppmann N, Graetz C, Paterka M, Poisa-Beiro L, Larochelle C, Hasan M, Lill CM, Zipp F, Siffrin V |
| Citation(s) |
25665584, 26612827 |
| |
| Submission date |
Apr 25, 2014 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Volker Siffrin |
| Organization name |
University Medical Center of the Johannes Gutenberg-University Mainz, Germany
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| Department |
Department of Neurology
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| Lab |
Immune dynamics in the CNS
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| Street address |
Langenbeckstrasse 1
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| City |
Mainz |
| ZIP/Postal code |
55131 |
| Country |
Germany |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA245422 |
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