|
| Status |
Public on Jan 08, 2007 |
| Title |
Changes in Gene Expression in Brassica oleracea Shoots during Phosphate Starvation (Cross-species study) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim of this study is to study gene expression in Brassica oleracea in shoot tissues of plants grown under contrasting P supplies (see Hammond JP et al., 2003, Plant Physiology, 132, 578-596 for background). Seeds of B. oleracea (var. alboglabra, A12dH) were first washed in 70% (v/v) ethanol/water, rinsed in distilled water and surface sterilised using 50% (v/v) domestic bleach/water. Seeds were rinsed and imbibed for 3 to 5 days in sterile distilled water at 4°C to break dormancy. Following imbibition, B. oleracea seeds were sown in un-vented, polycarbonate culture boxes (Sigma-Aldrich Company Ltd., Dorset UK). Seedlings were grown for 21 days on perforated polycarbonate discs (diameter 91 mm by 5 mm) placed on 75 ml of 0.8% (w/v) agar containing 1% (w/v) sucrose and a basal salt mix. Roots grew into the agar, but shoots remained on the opposite side of the disc. After 21 days, seedlings were transferred, still on polycarbonate discs, to a hydroponics system situated in a Saxcil growth cabinet (16 h daylength, set to 22°C and 80% humidity). Each polycarbonate disc was placed on a light-proof 500 ml beaker over 450 ml of nutrient solution. After 7 days, half the plants were transferred to nutrient solution containing no phosphate and the other half remained on full nutrient solution (control plants). Shoots were harvested 100 h after the withdrawal of P. !Samples were snap frozen in liquid nitrogen. RNA was extracted using the TRIzol extraction method and cleaned through a Qiagen RNeasy column.
Experimenter name = Martin Broadley
Experimenter phone = 0115 951 6382
Experimenter fax = 0115 951 6334
Experimenter institute = University of Nottingham
Experimenter address = Plant Sciences Division
Experimenter address = School of Biosciences
Experimenter address = University of Nottingham
Experimenter address = Sutton Bonington
Experimenter address = Loughborough
Experimenter zip/postal_code = LE12 5RD
Experimenter country = UK
Keywords: growth_condition_design
|
| |
|
| Overall design |
6 samples were used in this experiment
|
| |
|
| Contributor(s) |
Broadley M, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 25, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (6)
|
| GSM131103 |
Broadley_1-1_A1-Bo+P-nutrient-replete_Rep1_ATH1 |
| GSM131104 |
Broadley_1-2_A2-Bo-P-phosphate-starved_Rep1_ATH1 |
| GSM131105 |
Broadley_1-3_A3-Bo-P2-phosphate-starved_Rep2_ATH1 |
| GSM131106 |
Broadley_1-4_A4-Bo-P3-phosphate-starved_Rep3_ATH1 |
| GSM131107 |
Broadley_1-5_A5-Bo+P2-nutrient-replete_Rep2_ATH1 |
| GSM131108 |
Broadley_1-6_A6-Bo+P3-nutrient-replete_Rep3_ATH1 |
|
| Relations |
| BioProject |
PRJNA96907 |
Less...
More...