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| Status |
Public on Jul 03, 2014 |
| Title |
Heterochromatin-associated interactions of Drosophila HP1 with dADD1, HIPP1, and repetitive RNAs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We report chromatin-associated protein and RNA interactions of HP1a, indentified by BioTAP-XL mass spectrometry and sequencing. We identify an extensive list of both known and novel HP1a-interacting proteins from Drosophila S2 cells and from whole organisms across embryonic, larval and adult stages.
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| Overall design |
BioTAP-XL protocol was used to identify HP1a interacting proteins through mass-spectrometry analysis. ChIP-seq-like pulldown/input samples were generated using BioTAP-XL to validate genomic distribution of the HP1a-BioTAP contructs, and the newly identified interacting proteins (CG8290 and CG3680); To examine the RNAs associated with the HP1a complexes, the RNA fraction of the BioTAP-XL pulldowns was analyzed using Illumina-based RNA-seq protocols, as well as Helicos direct RNA sequencing.
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| Contributor(s) |
Kuroda MI, Alekseyenko AA, Kharchenko PV |
| Citation(s) |
24990964 |
| |
| Submission date |
Mar 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter Kharchenko |
| Organization name |
Harvard Medical School
|
| Department |
DBMI
|
| Lab |
Kharchenko
|
| Street address |
10 Shattuck St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (3) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL18532 |
Helicos HeliScope (Drosophila melanogaster) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA243597 |
| SRA |
SRP040969 |
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