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| Status |
Public on Mar 20, 2014 |
| Title |
Transcriptional analysis of live and hybridized J1 and NIH-3T3 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
J1 mouse embryonic stem cells (mESCs) and NIH-3T3 fibroblasts were grown in standard media conditions. Hybridized cells were fixed with 4% paraformaldehyde; permeabilized in 70% ethanol; incubated for 12 hours at 30C in an RNA preserving hybridization (RPH) buffer (300 mM Sodium chloride, 30mM Sodium citrate, 2.1M Ammonium sulfate, 25% formamide, 10 mM EDTA, 1 mg/ml E. Coli tRNA, 500 μg/ml BSA); and reverse cross-linked for 1 hour at 50C with Sodium dodecyl sulfate (SDS) and Proteinase K (100 mM NaCl, 10 mM Tris pH 8.0, 1 mM EDTA, 0.5% SDS, 500 μg/ml Proteinase K).
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| Overall design |
The fold change between J1 and NIH-3T3 cells was compared for live and hybridized samples (one replicate per sample).
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| Contributor(s) |
Klemm S, van Oudenaarden A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 14, 2014 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Sandy Klemm |
| E-mail(s) |
[email protected]
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
van Oudenaarden
|
| Street address |
31 Ames St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA241310 |
