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Series GSE5525

Query DataSets for GSE5525

Status

Public on Aug 15, 2006

Title

Transcriptome changes of Arabidopsis during pathogen and insect attack

Organism

Arabidopsis thaliana

Experiment type

Expression profiling by array

Summary

Plant defenses against pathogens and insects are regulated differentially by cross-communicating signaling pathways in which salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) play key roles. To understand how plants integrate pathogen- and insect-induced signals into specific defense responses, we monitored the dynamics of SA, JA, and ET signaling in Arabidopsis after attack by a set of microbial pathogens and herbivorous insects with different modes of attack. Arabidopsis plants were exposed to a pathogenic leaf bacterium (Pseudomonas syringae pv. tomato), a pathogenic leaf fungus (Alternaria brassicicola), tissue-chewing caterpillars (Pieris rapae), cell-content-feeding thrips (Frankliniella occidentalis), or phloem-feeding aphids (Myzus persicae). Monitoring the signal signature in each plant-attacker combination showed that the kinetics of SA, JA, and ET production varies greatly in both quantity and timing. Analysis of global gene expression profiles demonstrated that the signal signature characteristic of each Arabidopsis-attacker combination is orchestrated into a surprisingly complex set of transcriptional alterations in which, in all cases, stress-related genes are overrepresented. Comparison of the transcript profiles revealed that consistent changes induced by pathogens and insects with very different modes of attack can show considerable overlap. Of all consistent changes induced by A. brassicicola, P. rapae, and F. occidentalis, more than 50% were also induced consistently by P. syringae. Notably, although these four attackers all stimulated JA biosynthesis, the majority of the changes in JA-responsive gene expression were attacker-specific. All together our study shows that SA, JA, and ET play a primary role in the orchestration of the plant's defense response, but other regulatory mechanisms, such as pathway cross-talk or additional attacker-induced signals, eventually shape the highly complex attacker-specific defense response.
In the experimental set up we intended to select for genes that showed a more than 2-fold change in the same direction(up or down)at two time points after pathogen or insect attack.
The results are described in the following paper:
De Vos, M., Van Oosten, V.R., Van Poecke, R.M.P., Van Pelt, J.A., Pozo, M.J., Mueller, M.J., Buchala, A.J., Metraux, J.-P., Van Loon, L.C., Dicke, M. and Pieterse, C.M.J. (2005). Signal signature and transcriptome changes in Arabidopsis upon pathogen and insect attack. Molecular Plant-Microbe Interactions.

Experimenter name = Corne Pieterse
Experimenter phone = +31 30 253 3013
Experimenter fax = +31 30 251 8366
Experimenter department = Section Phytopathology
Experimenter institute = Utrecht University
Experimenter address = Department of Biology
Experimenter address = Utrecht University
Experimenter address = Sorbonnelaan 16
Experimenter address = Utrecht
Experimenter zip/postal_code = 3584CA
Experimenter country = The Netherlands
Keywords: time_series_design; pathogenicity_design

Overall design

14 samples were used in this experiment

Contributor(s)

Pieterse C, Townsend H, Emmerson Z, Schildknecht B

Citation(s)

16167763

Submission date

Aug 15, 2006

Last update date

Aug 28, 2018

Contact name

Nottingham Arabidopsis Stock Centre (NASC)