|
| Status |
Public on Aug 15, 2006 |
| Title |
Pieterse_1-5_avrPstDC3000-12h_Rep1_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Col-0
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Stock Code: N1092
Developmental stage: 3.90
|
| Treatment protocol |
P. syringae pv. tomato DC3000 with the plasmid pV288 carrying avirulence gene avrRpt2 (Kunkel et al., 1993) was cultured overnight at 28°C in liquid King's medium B, supplemented with 25 mg.L-1 kanamycin to select for the plasmid. Subsequently, bacterial cells were collected by centrifugation and resuspended in 10 mM MgSO4 to a final density of 107 cfu.mL-1. Wild-type Col-0 plants were inoculated by pressure infiltrating a suspension of P. syringae pv. tomato DC3000(avrRpt2) at 107 cfu.mL-1 into all fully expanded leaves of 5-week-old plants.
|
| Extracted molecule |
total RNA |
| Label |
biotin
|
| |
|
| Description |
Growth protocol: Seeds were sown in quartz sand for two weeks. Two-week-old seedlings were transferred to 60-mL pots containing a sand - potting soil mixture that was autoclaved twice for 20 min. Plants were cultivated in a growth chamber with a 8-h day (200 uEm-2.sec-1 at 24oC) and 16-h night (20oC) cycle at 70% relative humidity for another 3 weeks. Plants were watered every other day and received half-strength Hoagland nutrient solution containing 10 uM Sequestreen once a week.
|
| Data processing |
The data were analysed using Affymetrix Microarray Suite 5.0 (MAS5)
|
| |
|
| Submission date |
Aug 15, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE5525 |
Transcriptome changes of Arabidopsis during pathogen and insect attack |
|
| Relations |
| Reanalyzed by |
GSE119083 |
