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| Status |
Public on Nov 12, 2014 |
| Title |
Expression profiling of stroma cells from prostate cancer co-cultures, with or without TGF-beta signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Prostate stroma-specific TGF-beta signaling induces morphological changes in LNCaP cells. We have previously shown that stromal TGF-beta signaling regulates prostate tumor growth. To further delineate the underlying mechanisms, we generated LNCaP cells overexpressing an HA-tagged constitutively activate TGF-beta1 ligand (LNCaP-HA-TGF-β1(a)) and control LNCaP cells (LNCaP-Ctrl), and performed in vitro co-cultures of LNCaP-HA-TGF-β1(a) and LNCaP-Ctrl cells on top of the confluent HPS-19I cells, a human prostate stromal cell line. Since LNCaP cells are defective in TGF-beta receptor I (TbetaRI / ALK-5) that is essential for mediating TGF-beta signaling, only HPS19I cells are able to respond to TGF-beta ligand in these co-cultures. This provides a unique opportunity to study how prostate stromal cell-specific TGF-beta signaling regulates PCa biology. To identify the prostate epithelia-specific gene that was regulated by prostate stromal TGF-beta signaling, we also treated HPS19I cells using conditioned media collected from LNCaP- HA-TGF-β1(a) cells and LNCaP-Ctrl cells cultured in RPMI1640 supplemented with 0.2% FBS. After 6 days of treatment, we extracted total RNA from these HPS19I cells and performed microarray.
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| Overall design |
HPS-19I prostate stroma cells from LNCaP+HPS-19I co-culture, with or without TGF-beta
KEYWORDS: two group comparison
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| Contributor(s) |
Creighton C, Rowley D, Yang F |
| Citation(s) |
25333263 |
| |
| Submission date |
Oct 23, 2013 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| GSM1249451 |
HPS-19I cells from LNCaP+HPS-19I co-culture, no TGF-beta (36024_4) |
| GSM1249452 |
HPS-19I cells from LNCaP+HPS-19I co-culture, no TGF-beta (3926_4) |
| GSM1249453 |
HPS-19I cells from LNCaP+HPS-19I co-culture, with TGF-beta (36024_3) |
| GSM1249454 |
HPS-19I cells from LNCaP+HPS-19I co-culture, with TGF-beta (3926_3) |
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| This SubSeries is part of SuperSeries: |
| GSE51624 |
Co-cultures of prostate cancer cells and stroma cells, with or without TGF-beta signaling |
|
| Relations |
| BioProject |
PRJNA224106 |
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