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| Status |
Public on Nov 12, 2014 |
| Title |
Expression profiling of co-cultures of prostate cancer cells and stroma cells, with or without TGF-beta signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Prostate stroma-specific TGF-beta signaling induces morphological changes in LNCaP cells. We have previously shown that stromal TGF-beta signaling regulates prostate tumor growth. To further delineate the underlying mechanisms, we generated LNCaP cells overexpressing an HA-tagged constitutively activate TGF-beta1 ligand (LNCaP-HA-TGF-β1(a)) and control LNCaP cells (LNCaP-Ctrl), and performed in vitro co-cultures of LNCaP-HA-TGF-β1(a) and LNCaP-Ctrl cells on top of the confluent HPS-19I cells, a human prostate stromal cell line. Since LNCaP cells are defective in TGF-beta receptor I (TbetaRI / ALK-5) that is essential for mediating TGF-beta signaling, only HPS19I cells are able to respond to TGF-beta ligand in these co-cultures. This provides a unique opportunity to study how prostate stromal cell-specific TGF-beta signaling regulates PCa biology.
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| Overall design |
LNCaP + HPS19I co-cultures, with or without TGF-beta and with or without SD208 (a TBetaRI (ALK-5) protein kinase-specific inhibitor)
KEYWORDS: multiple group comparison
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| |
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| Contributor(s) |
Creighton C, Rowley D, Yang F |
| Citation(s) |
25333263 |
| |
| Submission date |
Oct 23, 2013 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (8)
|
| GSM1249402 |
LNCaP+HPS-19I co-culture, no TGF-beta or SD208 (36023_4) |
| GSM1249403 |
LNCaP+HPS-19I co-culture, no TGF-beta but with SD208 (36023_2) |
| GSM1249404 |
LNCaP+HPS-19I co-culture,with TGF-beta and DMSO (36023_3) |
| GSM1249405 |
LNCaP+HPS-19I co-culture,with both TGF-beta and SD208 (36023_1) |
| GSM1249406 |
LNCaP+HPS-19I co-culture, no TGF-beta or SD208 (33925_4) |
| GSM1249407 |
LNCaP+HPS-19I co-culture, no TGF-beta but with SD208 (33925_2) |
| GSM1249408 |
LNCaP+HPS-19I co-culture,with TGF-beta and DMSO (33925_3) |
| GSM1249409 |
LNCaP+HPS-19I co-culture,with both TGF-beta and SD208 (33925_1) |
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| This SubSeries is part of SuperSeries: |
| GSE51624 |
Co-cultures of prostate cancer cells and stroma cells, with or without TGF-beta signaling |
|
| Relations |
| BioProject |
PRJNA224104 |
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