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| Status |
Public on Aug 04, 2014 |
| Title |
Molecular characterization of 4-NQO induced F344 rat tongue carcinogenesis: alteration of multiple gene expression and hypomethylation of PTGS2 proximal promoter |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The 4-NQO induced rat tongue carcinogenesis model presenting considerable histologic and molecular similarity to oral cancers commonly seen in humans is very useful for investigating oral carcinogenesis. In order to understand the molecular basis of oral carcinogenesis and identify gene biomarkers and potential chemopreventive targets for future studies, we characterized the molecular changes in oral squamous cell carcinoma (SCC) samples generated from this model system using F344 rats by performing gene expression microarray and methylation analysis of selected genes. Microarray studies identified 1735 genes to be upregulated by at least 2 fold (p<0.05, n=11) and 1803 genes to be downregulated by at least 50% (p<0.05, n=11) in SCC in comparison to the adjacent normal tissues and 28 KEGG pathways to be altered in SCC (p<0.01). Among the altered genes, keratins and keratin associated proteins were found to be differentially regulated and the keratin profile appeared to an important biomarker for oral SCC. PTGS2 and PTGS2 relevant genes, which are potential targets of chemoprevention and therapy, were also found to be upregulated in SCC and confirmed by qRT-PCR. The upregulation of PTGS2 appeared to correlate with hypomethylation of its proximal promoter. Methylation analysis of other selected genes showed that the first exon of APC2 was methylated in normal tissues, and the methylation level increased moderately in SCC samples (p<0.01, n=8). These results demonstrate that 4-NQO induced tongue carcinogenesis in F344 rats is accompanied by alteration of multiple gene expression, hypomethylation of PTGS2 and increased methylation of APC2.
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| Overall design |
Gene expression in untreated normal vs.4-NQO induced tongue tumors in F344 rats (n=11).
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| Contributor(s) |
Peng X, Johnson WD, Li W, Torres KE, McCormick DL |
| Citation(s) |
25635769, 26516762 |
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| Submission date |
Sep 24, 2013 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Michael Falduto |
| E-mail(s) |
[email protected]
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| Phone |
847-291-9602
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| Organization name |
GenUs BioSystems, Inc.
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| Street address |
1808 Janke, Unit M
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| City |
Northbrook |
| State/province |
IL |
| ZIP/Postal code |
60062 |
| Country |
USA |
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| Platforms (1) |
| GPL14746 |
Agilent-028282 Whole Rat Genome Microarray 4x44K v3 (Probe Name version) |
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| Samples (22)
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| GSM1239148 |
Untreated Animal #13, Normal |
| GSM1239149 |
Untreated Animal #16, Normal |
| GSM1239150 |
Untreated Animal #17, Normal |
| GSM1239151 |
Untreated Animal #21, Normal |
| GSM1239152 |
Untreated Animal #22, Normal |
| GSM1239153 |
Untreated Animal #24, Normal |
| GSM1239154 |
Untreated Animal #25, Normal |
| GSM1239155 |
Untreated Animal #26, Normal |
| GSM1239156 |
Untreated Animal #4, Tumor |
| GSM1239157 |
Untreated Animal #9, Tumor |
| GSM1239158 |
Untreated Animal #11, Tumor |
| GSM1239159 |
Untreated Animal #13, Tumor |
| GSM1239160 |
Untreated Animal #16, Tumor |
| GSM1239161 |
Untreated Animal #17, Tumor |
| GSM1239162 |
Untreated Animal #21, Tumor |
| GSM1239163 |
Untreated Animal #22, Tumor |
| GSM1239164 |
Untreated Animal #24, Tumor |
| GSM1239165 |
Untreated Animal #25, Tumor |
| GSM1239166 |
Untreated Animal #26, Tumor |
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| Relations |
| BioProject |
PRJNA221235 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51125_RAW.tar |
195.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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