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| Status |
Public on Apr 20, 2006 |
| Title |
Transcript profiling in A. pleuropneumoniae under iron restriction |
| Organism |
Actinobacillus pleuropneumoniae |
| Experiment type |
Expression profiling by array
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| Summary |
To better understand effects of iron starvation on A. pleuropneumoniae and to identify new potential vaccine targets, we conducted transcript profiling using a DNA microarray containing all 2025 ORFs of the genome of A. pleuropneumoniae serotype 5b strain L20. Upon comparing growth of this pathogen in iron-sufficient versus iron-depleted medium, 210 genes were identified as being differentially expressed. Some genes (92) were identified as being up-regulated; many have confirmed or putative roles in iron acquisition, such as the genes coding for two TonB energy-transducing proteins and the hemoglobin receptor HgbA. Transcript profiling also led to identification of some new iron acquisition systems of A. pleuropneumoniae.
Keywords: stress respsonse
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| Overall design |
The bacteria was grown in BHI broth with or without 50 ug/ml EDDHA to generate iron restriction. RNA extractions were performed on five different days using the QIAGEN RNeasy Kit, and a total of nine hybridization were performed, including three pairs of dye swap experiments and a pair of hybridizations being a direct repeat (same dyes).
Data were acquired using a Perkin-Elmer ScanArray Express scanner, and were quantified, normalized and analysed using the SpotFinder, MIDAS and TMEV software from TIGR's TM4 Suite of Microarray Analysis softwares.
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| Contributor(s) |
Deslandes V, Nash JH, Harel J, Coulton JW, Jacques M |
| Citation(s) |
17355629 |
| |
| Submission date |
Apr 14, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Vincent Deslandes |
| E-mail(s) |
[email protected]
|
| Organization name |
Université de Montréal - Faculté de Médecine Vétérinaire
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| Department |
Groupe de Recherche sur les Maladies Infectieuses du Porc
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| Street address |
3200 rue Sicotte
|
| City |
St-Hyacinthe |
| State/province |
Quebec |
| ZIP/Postal code |
J2S7C6 |
| Country |
Canada |
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| Platforms (1) |
| GPL3648 |
AppChip1 (NRC-IBS Actinobacillus pleuropneumoniae L20) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA96775 |
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