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| Status |
Public on Mar 01, 2013 |
| Title |
Expression profile of S. pombe cells with ribosomal protein L32 paralogs |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
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| Summary |
We cultured the cells of S. pmbe in EMM2, and harvested them in the OD600 of 0.5 and 10 respectively.
We used microarrays to detail the the global program of gene expression of SP-Q01 and SP-Q01 carrying vevtor pESP3X to overexpress either ribosomal protein L32 paralog in the OD600 of 0.5 and 10 respectively.
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| Overall design |
We constructed the ribosomal protein L32 paralog overexpression strains to mimic the expression pattern of RPL32 paralogs in wild-type cells of S.pombe. We cultured the cells to different growth phase, and used microarray to identify the genes were regulated distinctively by RPL32 paralogs.
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| Contributor(s) |
Sun L, Yuan S |
| Citation(s) |
23577148 |
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| Submission date |
Jan 28, 2013 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Lei Sun |
| E-mail(s) |
[email protected]
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| Organization name |
Nanjing Normal University
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| Street address |
1 Wenyuan Rd
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| City |
Nanjing |
| ZIP/Postal code |
210046 |
| Country |
China |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (18)
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| GSM1071781 |
pombe 10 WT, biological rep1 |
| GSM1071782 |
pombe 10 WT, biological rep2 |
| GSM1071783 |
pombe 10 WT, biological rep3 |
| GSM1071784 |
pombe 0.5 RPL3201, biological rep1 |
| GSM1071785 |
pombe 0.5 RPL3201, biological rep2 |
| GSM1071786 |
pombe 0.5 RPL3201, biological rep3 |
| GSM1071787 |
pombe 0.5 RPL3202, biological rep1 |
| GSM1071788 |
pombe 0.5 RPL3202, biological rep2 |
| GSM1071789 |
pombe 0.5 RPL3202, biological rep3 |
| GSM1071790 |
pombe 10 RPL3201, biological rep1 |
| GSM1071791 |
pombe 10 RPL3201, biological rep2 |
| GSM1071792 |
pombe 10 RPL3201, biological rep3 |
| GSM1071793 |
pombe 10 RPL3202, biological rep1 |
| GSM1071794 |
pombe 10 RPL3202, biological rep2 |
| GSM1071795 |
pombe 10 RPL3202, biological rep3 |
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| Relations |
| BioProject |
PRJNA187580 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43827_RAW.tar |
16.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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