|
| Status |
Public on Mar 01, 2013 |
| Title |
pombe 10 RPL3202, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
S. pombe SP-Q01 harboring vector pESP3 with RPL3202 overexpression cultured in EMM2 medium to OD600 of 10
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
strain: SP-Q01/pESP3-RPL3202
growth phase: OD600 of 10
|
| Treatment protocol |
Cells were cellected and frozen in liquid nitrogen quickly for extraction of total RNA.
|
| Growth protocol |
The yeast strains were cultured in EMM2 medium at 30C, and cells were harvested when the OD600 was 0.5 and 10 respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (3_ivt_express_kit_manual Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 7G.
|
| Description |
Gene expression profile of S. pombe SP-Q01 harboring vector pESP3 with RPL3202 overexpression cultured in EMM2 medium to OD600 of 10
|
| Data processing |
The data were analyzed with PARTEK genomic suit 6.4 using RMA normalization
|
| |
|
| Submission date |
Jan 28, 2013 |
| Last update date |
Mar 01, 2013 |
| Contact name |
Lei Sun |
| E-mail(s) |
[email protected]
|
| Organization name |
Nanjing Normal University
|
| Street address |
1 Wenyuan Rd
|
| City |
Nanjing |
| ZIP/Postal code |
210046 |
| Country |
China |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE43827 |
Expression profile of S. pombe cells with ribosomal protein L32 paralogs |
|
