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| Status |
Public on Jan 04, 2013 |
| Title |
Expression data from non sexual flocculation cells of S. pombe |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
|
| Summary |
Deleting ribosomal protein genes caused haploid cells of S. pombe flocculated in full nutrition YEPD medium. We deleted rpl32-1 and rpl32-2 gene respectively and these two mutant cells cultured in YEPD medium flocculated.
We used microarrays to detail the global programme of gene expression between non flocculated rpl32-1 deleted and rpl32-2 deleted, compared with the non flocculated cells SPQ-01, and identified distinct classes of up-regulated genes during this process.
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| Overall design |
Yeast cells cultured in YEPD medium in the log phase were selected for RNA extraction and hybridization on Affymetrix microarrays. The rpl32-1 deleted cells were conformed as SD1 and rpl32-2 deleted cells were conformed as SD2. Control cells SPQ-01 were conformed as WT.
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| Contributor(s) |
Li R, Yuan S |
| Citation(s) |
23355005 |
| |
| Submission date |
Jan 02, 2013 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Rongpeng Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Nanjing normal university
|
| Department |
College of life science
|
| Street address |
Wenyuan Rd 1
|
| City |
Nanjing |
| ZIP/Postal code |
210046 |
| Country |
China |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA185196 |
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