|
| Status |
Public on Jan 04, 2013 |
| Title |
pombe_SD1,biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
S. pombe SD1 in log phase cultured in YEPD medium
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
strain: SPQ-01 with rpl32-1 deleted
|
| Treatment protocol |
Cells were collected and frozen in liquid nitrogen quickly for extraction of total RNA.
|
| Growth protocol |
The rpl32-1, rpl32-2 deleted and SPQ-01 yeast strains were cultured in YEPD medium at 30C, until the OD600 was 5.0.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (3_ivt_express_kit_manual Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 7G.
|
| Description |
Gene expression data from S. pombe SD1 in log phase cultured in YEPD medium
|
| Data processing |
The data were analyzed with PARTEK genomic suit 6.5 using RMA normalization
|
| |
|
| Submission date |
Jan 02, 2013 |
| Last update date |
Jan 04, 2013 |
| Contact name |
Rongpeng Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Nanjing normal university
|
| Department |
College of life science
|
| Street address |
Wenyuan Rd 1
|
| City |
Nanjing |
| ZIP/Postal code |
210046 |
| Country |
China |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE43248 |
Expression data from non sexual flocculation cells of S. pombe |
|
