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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 30, 2013 |
| Title |
Gene expression profiling of mature dendritic cells from mice s. c. treated with GM-CSF-gene transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Irradiated granulocyte macrophage-colony stimulating factor (GM-CSF)-transduced autologous tumor cells induce substantial antitumor immunity through the maturation and migration of dendritic cells (DCs). However, little is known about the key molecules involved in GM-CSF-sensitized DCs (GM-DCs) in tumor draining lymph nodes (TDLNs). We initially confirmed that mice subcutaneously injected with poorly immunogenic syngeneic Lewis lung carcinoma (LLC) cells transduced with Sendai virus encoding GM-CSF (LLC/SeV/GM) significantly rejected the tumor growth. Using microarray expression profiling, we obtained a large number of gene expression data files from GM-DCs and control DCs in TDLNs, and subjected them to network-based cluster analysis and unexpectedly unraveled the expression levels of type I IFNs-related genes specifically expressed in plasmacytoid DCs (pDC) were robustly up-regulated in GM-DCs. In vivo depletion assay showed that pDC-depleted mice treated with subcutaneous LLC/SeV/GM cells abrogated the antitumor effects observed in control mice. Moreover combination use of imiquimod for TLR7 triggering on pDC with irradiated LLC/SeV/GM cells induced a significant therapeutic antitumor effect with marked induction of CD9+ pDC with antitumor phenotype, whereas other control mice groups had only minimal to-modest antitumor responses, implicating that this combined vaccine strategy using imiquimod could be promising for improvement of GM-CSF-induced antitumor immunity.
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| Overall design |
Mouse GM-CSF induced gene expression in mature dendritic cells in tumor draining lymph nodes from C57/BL6N female mouse was measured at 2 days after s.c. tumor challenge with GM-CSF gene-transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP).
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| Contributor(s) |
Narusawa M, Inoue H, Sakamoto C, Inoue T, Watanabe A, Miyamoto S, Miura Y, Hijikata Y, Inoue M, Takayama K, Okazaki T, Hasegawa M, Nakanishi Y, Tani K |
| Citation(s) |
24830413 |
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| Submission date |
Dec 27, 2012 |
| Last update date |
Nov 01, 2017 |
| Contact name |
kenzaburo tani |
| E-mail(s) |
[email protected]
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| Organization name |
Kyushu University
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| Department |
Medical Institute of Bioregulation
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| Lab |
Department of Molecular Genetics
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| Street address |
3-1-1 Maidashi
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| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA184817 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43169_RAW.tar |
26.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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