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Status |
Public on Oct 04, 2012 |
Title |
BG3 WT Repliseq |
Project |
modENCODE
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Organism |
Drosophila melanogaster |
Experiment type |
Other
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Summary |
modENCODE_submission_5528 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The relative time of replication for all unique sequences in the Drosophila genome was determined by sequencing replication intermediates that were differentially labeled with BrdU during S-phase progression. Briefly, cells were pulse labeled with BrdU and sorted into early, middle and late S-phase fractions. BrdU labeled sequences from each fraction of S-phase were enriched by immunoprecipitation and sequenced on the Illumina platform. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
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Overall design |
EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; EXPERIMENTAL FACTORS: read length (base pair) ; Cell Line ML-DmBG3-c2
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Web link |
http://www.modencode.org http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
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Contributor(s) |
MacAlpine D, Lubelsky Y, Belsky J, Prinz J |
Citation(s) |
24985913 |
BioProject |
PRJNA63463 |
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Submission date |
Oct 04, 2012 |
Last update date |
May 15, 2019 |
Contact name |
DCC modENCODE |
E-mail(s) |
[email protected]
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Phone |
416-673-8579
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Organization name |
Ontario Institute for Cancer Research
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Lab |
modENCODE DCC
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Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
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City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5G 0A3 |
Country |
Canada |
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Platforms (1) |
GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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Samples (8)
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GSM1015358 |
ML-DmBG3-c2 WT Early S-phase Replicate 1 extraction1_seq1 aliquote 1 |
GSM1015359 |
ML-DmBG3-c2 WT Mid-Early S-phase Replicate 1 extraction2_seq1 aliquote 1 |
GSM1015360 |
ML-DmBG3-c2 WT Mid-Late S-phase Replicate 1 extraction3_seq1 aliquote 1 |
GSM1015361 |
ML-DmBG3-c2 WT LateSphase Replicate 1 extraction4_seq1 aliquote 1 |
GSM1015362 |
ML-DmBG3-c2 WT Early S-phase Replicate 2 extraction5_seq1 aliquote 1 |
GSM1015363 |
ML-DmBG3-c2 WT Mid-Early S-phase_Replicate 2 extraction6_seq1 aliquote 1 |
GSM1015364 |
ML-DmBG3-c2 WT Mid-Late S-phase Replicate 2 extraction7_seq1 aliquote 1 |
GSM1015365 |
ML-DmBG3-c2 WT Late S-phase Replicate 2 extraction8_seq1 aliquote 1 |
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Relations |
SRA |
SRP016083 |