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| Status |
Public on Dec 04, 2013 |
| Title |
Microarray analysis of gene response to contaminant accumulation in wild largescale suckers |
| Platform organism |
Catostomus snyderi |
| Sample organism |
Catostomus macrocheilus |
| Experiment type |
Expression profiling by array
|
| Summary |
Toxic compounds such as organochlorine pesticides (OCs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ether flame retardants (PBDEs) have been detected in fish, birds, and aquatic mammals that live in the Columbia River or use the river as a food source. We developed a custom microarray for largescale suckers (Catostomus macrocheilus) and used it to investigate the molecular effects of contaminant exposure on wild fish in the Columbia River. Using Significance Analysis of Microarrays (SAM) we identified 72 probes representing 69 unique genes with expression patterns that correlated with hepatic tissue levels of OCs, PCBs, or PBDEs. These genes were involved in many biological processes previously shown to respond to contaminant exposure, including drug and lipid metabolism, apoptosis, cellular transport, oxidative stress, and cellular chaperone function. The relation between gene expression and contaminant burden suggests that these genes may respond to environmental contaminant exposure and are promising candidates for further field and laboratory studies to develop biomarkers for monitoring exposure of wild fish to contaminant mixtures found in the Columbia River Basin
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| Overall design |
Correlation between contaminant exposure and gene expression profiles of wild largescale suckers collected from three different sites in the Columbia River. At each site, liver samples of six to eight different fish were analyzed using microarrays.
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| Contributor(s) |
Christiansen HE, Mehinto AC, Yu F, Perry RW, Denslow ND, Maule AG, Mesa MG |
| Citation(s) |
24050789 |
| |
| Submission date |
Sep 19, 2012 |
| Last update date |
Mar 06, 2014 |
| Contact name |
Alvine C Mehinto |
| Organization name |
Southern California Coastal Water Research Project
|
| Lab |
Center for Envirom & Human Toxicol
|
| Street address |
3535 Harbor Blvd, Suite 110
|
| City |
Costa Mesa |
| ZIP/Postal code |
92626 |
| Country |
USA |
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| Platforms (1) |
| GPL16082 |
Agilent-035137 Catostomus snyderi array [Probe Name] |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA175537 |
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