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| Status |
Public on Sep 01, 2013 |
| Title |
RNA sequencing in fly heads to examine the effect of spermidine feeding on transcription in the ageing fly brain. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
mRNA sequencing was used to identify genome wide transcriptional changes occuring in fly heads in response to spermidine feeding. This study shed light on the molecular mechanisms through wich spermidine can protect against age-dependent memory impairment.
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| Overall design |
mRNA profiles from 3 and 10 day old Drosophila melanogaster heads were generated in duplicate by deep sequencing using Illumina GAIIx. mRNA profiles from flies that were fed food with 5mM spermidine were compared to profiles from flies that had no spermidine in thier food.
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| Contributor(s) |
Kramer JM, Koemans TS, Schenck A, Gupta VK, Scheunemann L, Eisenberg T, Mettke C, Mertel S, Bhuke A, Stunnenberg HG, Sinner F, Magnes C, Dipt S, Fiala A, Schwaerzel M, Madeo F, Sigris SJ |
| Citation(s) |
23995066 |
| |
| Submission date |
Jun 28, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Jamie M Kramer |
| E-mail(s) |
[email protected]
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| Organization name |
Radboud University Nijmegen Medical Centre
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| Street address |
Geert Grootplein 10
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| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
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| Platforms (2) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA169561 |
| SRA |
SRP014006 |
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