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| Status |
Public on Feb 06, 2013 |
| Title |
The BET protein FSH functionally interacts with ASH1 to orchestrate global gene activity in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Background: The question of how cells re-establish gene expression states after cell division is still poorly understood. Genetic and molecular analyses have indicated that Trithorax group (TrxG) proteins are critical for the long-term maintenance of active gene expression states in many organisms. A generally accepted model suggests that TrxG proteins contribute to maintenance of transcription by protecting genes from inappropriate Polycomb group (PcG)-mediated silencing, instead of directly promoting transcription. Results: Here we report a physical and functional interaction in Drosophila between two members of the TrxG, the histone methyltransferase ASH1 and the bromodomain and extraterminal family protein FSH. We investigated this interface at the genome level, uncovering a widespread colocalization of both proteins at promoters and PcG-bound intergenic elements. Our integrative analysis of chromatin maps and gene expression profiles revealed that the observed ASH1-FSH binding pattern at promoters is a hallmark of active genes. Inhibition of FSH-binding to chromatin resulted in global down-regulation of transcription. In addition, we found that genes displaying marks of robust PcG-mediated repression also have ASH1 and FSH bound to their promoters. Conclusions: Our data strongly favor a global coactivator function of ASH1 and FSH during transcription, as opposed to the notion that TrxG proteins impede inappropriate PcG-mediated silencing, but are dispensable elsewhere. Instead, our results suggest that PcG repression needs to overcome the transcription-promoting function of ASH1 and FSH in order to silence genes.
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| Overall design |
Refer to individual Series
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| Contributor(s) |
Kockmann T, Gerstung M, Schlumpf T, Xhinzhou Z, Hess D, Beerenwinkel N, Beisel C, Paro R |
| Citation(s) |
23442797 |
| |
| Submission date |
Mar 12, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Tobias Kockmann |
| E-mail(s) |
[email protected]
|
| Organization name |
ETHZ
|
| Department |
D-BSSE
|
| Lab |
Paro
|
| Street address |
Mattenstr. 26
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
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| Platforms (2) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (16)
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| This SuperSeries is composed of the following SubSeries: |
| GSE36180 |
Effects of pharmacologic FSH inhibition on gene expression |
| GSE36404 |
Generation of chromatin maps for ASH1 and FSH applying ChIP-seq |
| GSE43180 |
Effects of FSH knockdown on gene expression |
|
| Relations |
| BioProject |
PRJNA153201 |
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