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| Status |
Public on Nov 30, 2011 |
| Title |
Hepatic gene expression profiles after long-term high-fat diet feeding in wild type and Nrf2 knock-out mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To assess the role of the transcription factor NFE2 related factor 2 like 2 ( Nrf2) in the development of high-fat diet (HFD)-induced obesity and non-alcoholic HFD-induced fatty liver disease, 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) C57BL6J male mice (obtained from Riken BRC, Tsukuba, Japan and originally developed by Prof. M. Yamamoto) were fed an HFD (60 kcal % fat) for 180 days. Whole genome microarray expression profiling was performed in pooled liver samples of WT and Nrf2-KO mice to identify genes that are differentially expressed between WT and Nrf2-KO mice under the stress conditions of HFD-induced obesity.
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| Overall design |
Liver samples were taken from 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) male mice on high-fat diet (60 kcal % fat) for 180 days. Total RNA was isolated from pooled liver samples from WT or Nrf2-KO mice to produce 4 samples each using the guanidinium thiocyanate method.
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| Contributor(s) |
Chartoumpekis D, Zaravinos A, Habeos I |
| Citation(s) |
23710285 |
| |
| Submission date |
Nov 09, 2011 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Dionysios Chartoumpekis |
| E-mail(s) |
[email protected]
|
| Organization name |
Lausanne University Hospital CHUV
|
| Department |
Service of Endocrinology Diabetes and Metabolism
|
| Street address |
Ave de la Sallaz 8
|
| City |
Lausanne |
| ZIP/Postal code |
1011 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (8)
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| GSM830131 |
Liver wild type High Fat Diet, replicate 1 |
| GSM830132 |
Liver wild type High Fat Diet, replicate 2 |
| GSM830133 |
Liver wild type High Fat Diet, replicate 3 |
| GSM830134 |
Liver wild type High Fat Diet, replicate 4 |
| GSM830135 |
Liver Nrf2 knock-out High Fat Diet, replicate 1 |
| GSM830136 |
Liver Nrf2 knock-out High Fat Diet, replicate 2 |
| GSM830137 |
Liver Nrf2 knock-out High Fat Diet, replicate 3 |
| GSM830138 |
Liver Nrf2 knock-out High Fat Diet, replicate 4 |
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| Relations |
| BioProject |
PRJNA148571 |
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