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| Status |
Public on Mar 12, 2012 |
| Title |
The response of murine primary airway epithelial cells to Influenza infection and the importance of Interferon type I signaling in this response [mAEC]. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate how murine airway epithelial cells respond to Influenza infection and how important interferon type I signaling is for this response, we harvested airway epithelial cells from the tracheas of wild type, interferon type I knockout(IFNaR-/-) and STAT1 knockout (STAT1-/-) mice and cultured them as previously described (Pickles et al,1998) in polarized airway epithelial cell cultures (mAECs). Triplicate mAECs from each type of mouse (wt,IFNaR-/-,STAT1-/-) were infected with 2X105 PFUs Influenza A (WSN) for 2h or mock inoculated and harvested 24h after infection.
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| Overall design |
Triplicate murine polarized airway epithelial cell cultures from wild type, IFNaR-/- or STAT1-/- mice were mock treated or infected with 2x10^5 PFUs of Influenza A (WSN) for 2h and harvested 24 h post infection.
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| Contributor(s) |
Ioannidis I, McNally B, Willette M, Flano E |
| Citation(s) |
22398282 |
| |
| Submission date |
Sep 15, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
IOANNIS IOANNIDIS |
| E-mail(s) |
[email protected]
|
| Phone |
614-355-3038
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| Organization name |
NATIONWIDE CHILDRENS HOSPITAL
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| Department |
VACCINES AND IMMUNITY
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| Lab |
FLANO LAB
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| Street address |
700 CHILDRENS DRIVE
|
| City |
COLUMBUS |
| State/province |
OH |
| ZIP/Postal code |
43220 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE32140 |
The response of PBMCs and primary airway epithelial cells to Influenza and RSV virus |
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| Relations |
| BioProject |
PRJNA154959 |
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