 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 10, 2012 |
| Title |
The Conserved Polarity Factor PodJ1 Impacts Multiple Cell Envelope-Associated Functions in Sinorhizobium meliloti |
| Platform organisms |
Sinorhizobium meliloti; Medicago truncatula |
| Sample organism |
Sinorhizobium meliloti |
| Experiment type |
Expression profiling by array
|
| Summary |
Although diminutive in size, bacteria possess highly diverse and spatially confined cellular structures. Two related alpha-proteobacteria, Sinorhizobium meliloti and Caulobacter crescentus, serve as models for investigating the genetic basis of morphologic variations. S. meliloti, a symbiont of leguminous plants, synthesizes multiple flagella and no prosthecae, whereas C. crescentus, a freshwater bacterium, has a single polar flagellum and stalk. The podJ gene, originally identified in C. crescentus for its role in polar organelle development, is split into two juxtaposed open reading frames, podJ1 and podJ2, in S. meliloti. Deletion of podJ1 interferes with flagellar motility, exopolysaccharide production, cell envelope integrity, cell division, and normal morphology, but not symbiosis. As in C. crescentus, the S. meliloti PodJ1 protein appears to act as a polarity beacon and localizes to the newer cell pole. Microarray analysis indicates that podJ1 affects the expression of at least 129 genes, the majority of which correspond to observed mutant phenotypes. The combination of phenotypic characterization, microarray analysis, and suppressor identification suggests that PodJ1 controls a core set of conserved elements, including flagellar and pili genes, the signaling proteins PleC and DivK, and the TacA transcriptional activator, while alternate downstream targets have evolved to suit the distinct lifestyles of individual species.
|
| |
|
| Overall design |
Gene expression profiling of Sinorhizobium meliloti Rm1021 or its isogenic podJ1 deletion mutant, grown to mid exponential phase in rich medium, was performed using custom Affymetrix GeneChips.
|
| |
|
| Contributor(s) |
Fields AT, Barnett MJ, Long SR, Chen JC |
| Citation(s) |
22553970 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM093628 |
Control of Symbiotic Gene Expression in Sinorhizobium meliloti |
STANFORD UNIVERSITY |
SHARON R LONG |
| SC2 GM082318 |
Regulated Assembly of Subcellular Structures in alpha-Proteobacteria |
SAN FRANCISCO STATE UNIVERSITY |
Joseph Chiung-Chu Chen |
|
| |
| Submission date |
Aug 10, 2011 |
| Last update date |
Feb 17, 2015 |
| Contact name |
Melanie J Barnett |
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Sharon R. Long
|
| Street address |
371 Jane Stanford Way
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9757 |
[stSmel_Mtra] SRL_SU Sinorhizobium meliloti/Medicago truncatula dual-genome SymbiosisChip, Affymetrix part number 510699 |
|
| Samples (6)
|
| GSM776975 |
Sinorhizobium meliloti podJ1 deletion mutant, biological replicate1 |
| GSM776976 |
Sinorhizobium meliloti podJ1 deletion mutant, biological replicate2 |
| GSM776977 |
Sinorhizobium meliloti podJ1 deletion mutant, biological replicate3 |
| GSM776978 |
Sinorhizobium meliloti Rm1021, biological replicate1 |
| GSM776979 |
Sinorhizobium meliloti Rm1021, biological replicate2 |
| GSM776980 |
Sinorhizobium meliloti Rm1021, biological replicate3 |
|
| Relations |
| BioProject |
PRJNA146015 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31325_RAW.tar |
23.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...