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| Status |
Public on Oct 30, 2014 |
| Title |
Identifying targets of DSX with DamID-seq |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We have adapted the DamID protocol for use with high throughput sequencing. We have used DamID to identify the positions within the Drosophila genome where the transcription factor DSX is bound. We sequenced DpnI-digested genomic DNA from fly tissues containing UAS-Dam (control) or UAS-Dam-DsxF or UAS-Dam-DsxM.
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| Overall design |
We have performed DamID-seq on adult male and female fatbody and on ovary. We used two biological replicates for each tissue and sex.
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| Contributor(s) |
Clough E, Jimenez E, Kim Y, Whitworth C, Neville M, Hempel L, Pavlou H, Chen Z, Sturgill D, Dale R, Smith HE, Przytycka TM, Goodwin S, Van Doren M, Oliver B |
| Citation(s) |
25535918 |
| |
| Submission date |
Feb 12, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian Oliver |
| E-mail(s) |
[email protected]
|
| Phone |
301-204-9463
|
| Organization name |
NIDDK, NIH
|
| Department |
LBG
|
| Lab |
Developmental Genomics
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| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE49480 |
Identifying targets of DSX with ChIP-seq, DamID-seq and DamID-chip and transcriptional response to DSX isoform switch |
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| Relations |
| SRA |
SRP005701 |
| BioProject |
PRJNA137151 |
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