|
| Status |
Public on May 24, 2024 |
| Title |
Loss of SIRPα promotes podocytes presenting antigen to activate specific T cell immune responses in lupus nephritis via activating spleen tyrosine kinase |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate the role of SIRPα in podocytes, we generated podocyte-specific SIRPα knockout mice, Subsequent studies have found that SIRPa is deeply involved in the adaptive immune process of podocytes, and the loss of SIRPa promotes the ability of podocytes to present antigens,furthermore, the loss of SIRPa in podocytes can promote the increase of antigen presentation ability of podocytes and promote the progression of lupus nephrin
|
| |
|
| Overall design |
The wild type (WT) and podocyte specific SIRPα knockout (Sirpa-/-) mice were received a single intraperitoneal injection of 0.5 ml pristane respectively, and monitored for 4 months to construct experimental LN mice. Podocytes were isolated from pristane-treated WT and Sirpa-/- mice for further analysis.
|
| |
|
| Contributor(s) |
Qian B, Li L |
| Citation(s) |
38758648 |
| |
| Submission date |
Aug 15, 2023 |
| Last update date |
May 24, 2024 |
| Contact name |
QIAN BIN |
| E-mail(s) |
[email protected]
|
| Phone |
15851836627
|
| Organization name |
State Key Laboratory of Natural Medicines
|
| Department |
School of Life Science and Technology
|
| Lab |
Jiangsu Key Laboratory of Druggability of Biopharmaceuticals
|
| Street address |
639 Longmian Avenue
|
| City |
Nanjing |
| State/province |
JiangSu |
| ZIP/Postal code |
211198 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA1005685 |
