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| Status |
Public on Apr 28, 2023 |
| Title |
Metabolic rescue of α-synuclein-induced neurodegeneration through propionate supplementation and intestine-neuron signaling in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Vitamin B12 depletion or propionate supplementation can reverse the transcriptomic aberration in the C. elegans model of PD with neuronal overexpression of alpha-synuclein A53T
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| Overall design |
C. elegans strains with pan-neuronal or dopaminergic neuron-specific overexpression of human alpha-synuclein A53T proteins were fed with either wild-type or B12-depleted cobS(-); cobA(-) K12 E. coli. The PD animals were also supplemented with either propionic acid or vitamin B12 to assess their effects on the transcriptome. Wild-type C. elegans carrying a transgene that expressed GFP in the dopaminergic neurons was used as a control strain. Animals were collected at L4 stage and subjected to whole-organism RNA sequencing. Ribosomal RNAs were depleted from the total RNA during the library construction.
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| Citation missing |
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| Submission date |
Apr 27, 2023 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Chaogu Zheng |
| E-mail(s) |
[email protected]
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| Organization name |
University of Hong Kong
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| Department |
School of Biological Science
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| Street address |
The University of Hong Kong, Pokfulam Road
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| City |
HK |
| ZIP/Postal code |
00000 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL25145 |
BGISEQ-500 (Caenorhabditis elegans) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA962567 |
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