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| Status |
Public on Jul 27, 2010 |
| Title |
Gene expression profile associated with the PDZ ligand-motif of HPV16-E6 oncoprotin in the skin of transgenic mice models |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Third-party reanalysis
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| Summary |
The K14E6 transgenic mice developskin hyperplasia,benign tumors and skin cancer. The tumor formation capacity diminishes when E6 lacks its PDZ ligand-motif.
This study aims to compare the transciptional profiles of transgenic K14E6 mice with K14E6 Δ146-151 (lacking the PDZ domain) in order to explore the role of PDZ in early skin carcinogenesis.
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| Overall design |
Skin tissue from three different strains of mice were dissected for RNA extraction, processing and hybridization in Affymetrix Mouse Genome 430 2,0 array. We processed three biological replicates per strain. 3 mice were used for each replicate pool for a final RNA concentration of 3 micrograms. The arrays were analyzed using Partek Genomics suite software.
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| Contributor(s) |
Roman-Rosales A, Bonilla-Delgado J, Garcia-Villa E, Uribe-Figueroa L, Rangel-Escareno C, Hidalgo-Miranda A, Lambert PF, Gariglio P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 17, 2010 |
| Last update date |
May 04, 2018 |
| Contact name |
Laura I Uribe |
| E-mail(s) |
[email protected]
|
| Phone |
+525553501909
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| Organization name |
INMEGEN
|
| Department |
Research
|
| Lab |
UGAE Affymetrix
|
| Street address |
Periferico Sur 4124 Col. Ex rancho de Anzaldo
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| City |
Mexico City |
| State/province |
DF |
| ZIP/Postal code |
01900 |
| Country |
Mexico |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (7)
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| GSM557240 |
K14E6 Δ146-151 transgenic mice RNA pool biological replicate 1 |
| GSM557241 |
K14E6 Δ146-151 transgenic mice RNA pool biological replicate 2 |
| GSM557242 |
K14E6 Δ146-151 transgenic mice RNA pool biological replicate 3 |
| GSM570062 |
K14E6 transgenic mice RNA pool biological replicate 2 |
| GSM570063 |
K14E6 transgenic mice RNA pool biological replicate 3 |
| GSM570064 |
FvB non transgenic control mice RNA pool biological replicate 2 |
| GSM570065 |
FvB non transgenic control mice RNA pool biological replicate 3 |
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| Relations |
| Reanalysis of |
GSM270601 |
| Reanalysis of |
GSM270603 |
| BioProject |
PRJNA128563 |
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