 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 28, 2010 |
| Title |
Expression data from Saccharomyces cerevisiae treated with Small Molecule Enhancers of Rapamycin (SMERs) |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
The target of rapamycin (TOR) plays a central role in eukaryotic cell growth control. With prevalent hyper-activation of the mTOR pathway in human cancers, novel strategies to enhance TOR pathway inhibition are highly desirable.
We used a yeast-based high-throughput chemical genetic screen to identify small-molecule enhancers of rapamycin (SMERs) and used whole genome expression analysis to identify their mechanisms of action.
|
| |
|
| Overall design |
We treated yeast individually with SMERs 1-5, rapamycin, or DMSO (solvent control) for 30 minutes prior to RNA extraction and hybridization on Affymetrix microarrays. Expression profiles of SMER-treated samples were compared to that of DMSO (solvent control) and rapamycin-treated samples to identify gene expression signatures unique to SMER-treated samples.
|
| |
|
| Contributor(s) |
Aghajan M, Jonai N, Flick K, Fu F, Luo M, Cai X, Ouni I, Pierce N, Tang X, Lomenick B, Damoiseaux R, Hao R, del Moral PM, Verma R, Li Y, Li C, Houk KN, Jung ME, Zheng N, Huang L, Deshaies RJ, Kaiser P, Huang J |
| Citation(s) |
20581845 |
| |
| Submission date |
Jun 10, 2010 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Mariam Aghajan |
| Organization name |
UCLA
|
| Street address |
650 Charles E. Young Drive South, CHS 23-231
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (13)
|
|
| Relations |
| BioProject |
PRJNA128725 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22269_RAW.tar |
13.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...