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| Status |
Public on Jul 31, 2023 |
| Title |
Evaluation of global gene expression changes in the larval intestines of PNGase-like (Pngl) mutant Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mutations in Drosophila Pngl lead to structural and functional abnormalities in the larval intestine. Here we report using RNA-sequencing of Drosophila larval intestines to determine the effects of loss of Pngl on the transcriptome in this tissue.
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| Overall design |
Third instar Drosophila larvae with the following four different genotypes were synchronized at 96 hours after egg laying: 1. yellow white (y w control), 2. y w; Pngl[ex14]/CyO (Pngl heterozygous), 3. y w; Pngl[ex14]/Pngl[ex14] (Pngl homozygous), and 4. y w; Pngl[ex14]/Pngl[ex14]; PBac{Pngl[wt]Ch322-3O13}VK27 (Pngl homozygous rescued by one copy of a Pngl genomic transgene).
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| Contributor(s) |
Story B |
| Citation(s) |
37704604 |
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| Submission date |
Jun 16, 2022 |
| Last update date |
Sep 21, 2023 |
| Contact name |
Benjamin Andrew Story |
| E-mail(s) |
[email protected]
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| Organization name |
European Molecular Biology Laboratory (EMBL)
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| Department |
Genomics
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| Street address |
Meyerhofstrasse 1
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| City |
Heidelberg |
| ZIP/Postal code |
69117 |
| Country |
Germany |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (8)
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| GSM6246432 |
y w, Pngl-ex14, Cyo replicate 2 |
| GSM6246433 |
y w, Pngl-ex14, Pngl-ex14, PBAC replicate 1 |
| GSM6246434 |
y w, Pngl-ex14, Pngl-ex14, PBAC replicate 2 |
| GSM6246435 |
y w, Pngl-ex14, Pngl-ex14 replicate 1 |
| GSM6246436 |
y w, Pngl-ex14, Pngl-ex14 replicate 2 |
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| Relations |
| BioProject |
PRJNA849773 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206229_counts.txt.gz |
217.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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